부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.envres.2005.08.007 Byline: Margot Lundborg (a), Sven-Erik Dahlen (a), Urban Johard (b), Per Gerde (a), Connie Jarstrand (c), Per Camner (a), Lena LA[yen]stbom (a) Keywords: Alveolar macrophages; Carbon particles; Diesel exhaust particles; Lung infections; Phagocytosis Abstract: We investigated whether exposure of alveolar macrophages to aggregates of ultrafine carbon particles affected subsequent phagocytosis of microorganisms. Human alveolar macrophages were obtained by bronchoalveolar lavage and exposed to aggregates of ultrafine carbon particles or diesel exhaust particles (DEP) for 20h before measurements of phagocytosis. The particle loads were estimated to be comparable to those of air pollution exposure with established health effects in humans. Phagocytotic activity was measured as attachment and ingestion of four different test particles (amorphous silica particles, yeast cells from Candida albicans, and Cryptococcus neoformans opsonized with specific IgG or fresh serum) that bind to scavenger, mannose, Fc, and complement receptors, respectively. Carbon preloading significantly impaired the attachment and ingestion process (P Author Affiliation: (a) Division of Physiology, The National Institute of Environmental Medicine, Karolinska Institutet, P.O. Box 287, SE-171 77 Stockholm, Sweden (b) Division of Respiratory Medicine, Department of Medicine at Karolinska University Hospital, Karolinska Institutet, Stockholm 77, Sweden (c) Division of Clinical Bacteriology, Department of Microbiology, Pathology and Immunology at Huddinge University Hospital, Karolinska Institutet, Stockholm 77, Sweden Article History: Received 14 December 2004; Revised 28 June 2005; Accepted 5 August 2005 Article Note: (footnote) [star] The study was supported by grants from the Swedish Heart-Lung Foundation, the European Commission (contract no. FIS5-1999-00214, BIODOS), the Swedish Environmental Protection Agency, the Swedish Medical Research Council (14X-0971) and Karolinska Institutet.