부산대학교 도서관

The phytosterol, tocopherol, and tocotrienol profiles for mkukubuyo, Sterculia africana, manketti, Ricinodendron rautanenni, mokolwane, Hyphaene petersiana, morama, Tylosema esculentum, and moretologa-kgomo, Ximenia caffra, seed oils from Botswana have been determined. Normal-phase HPLC analysis of the unsaponifiable matter showed that among the selected oils, the most abundant tocopherol and tocotrienol were I3-tocopherol (2232.99 ug/g) and I3-tocotrienol (246.19 ug/g), detected in manketti and mkukubuyo, respectively. Mokolwane oil, however, contained the largest total tocotrienol (258.47 ug/g). Total tocol contents found in manketti, mokolwane, mkukubuyo, morama, and moretologa-kgomo oils were 2238.60, 262.40, 246.20, 199.10, and 128.0 ug/g, respectively. GC--MS determination of the relative percentage composition of phytosterols showed 4-desmethylsterols as the most abundant phytosterols in the oils, by occurring up to 90% in moretologa-kgomo, mkukubuyo, and manketti seed oils, with [beta]-sitosterol being the most abundant. Mokolwane seed oil contained the largest percentage composition of 4,4-dimethylsterols (45.93%). Besides 4-desmethylsterols (75%), morama oil also contained significant amounts of 4,4-dimethylsterols and 4-monomethylsterols (15.72% total). GC--MS determination of the absolute amounts of 4-desmethylsterols, after SPE fractionation of the unsaponifiable matter, confirmed that [beta]-sitosterol was the most abundant phytosterol in the test seed oils, with manketti seed oil being the richest source (1326.74 ug/g). The analysis showed total 4-desmethylsterols content as 1617.41, 1291.88, 861.47, 149.15, and 109.11 ug/g for manketti, mokolwane, mkukubuyo, morama, and moretologa-kgomo seed oils, respectively.