학술논문
A method for acetonitrile-free microcystin analysis and purification by high-performance liquid chromatography, using methanol as mobile phase
Document Type
Academic Journal
Source
Toxicon. Nov, 2009, Vol. 54 Issue 6, p887, 4 p.
Subject
Language
English
ISSN
0041-0101
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.toxicon.2009.06.016 Byline: Esme L. Purdie, Fiona M. Young, Diana Menzel, Geoffrey A. Codd Abstract: Cyanobacteria produce a wide range of potent toxins, including hepatotoxic microcystins. HPLC methods for microcystin analysis and purification almost invariably include acetonitrile in the elution gradient mobile phase. The recent, acute, global acetonitrile shortage requires that adequate methods are available for microcystin analysis and purification without the need for acetonitrile. Here we present a convenient methanol-based method for effective HPLC analysis and purification of the toxins, with full separation of a range of microcystin variants. Author Affiliation: Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom Article History: Received 14 April 2009; Revised 11 June 2009; Accepted 16 June 2009