부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1600-051X.2006.00983.x Byline: D. Sakellari (1), V. Katsares (2), M. Georgiadou (2), A. Kouvatsi (2), M. Arsenakis (2), A. Konstantinidis (1) Keywords: COL1A1; Greek; IL1A; IL1B; IL1RN; periodontitis; SNP; TNFA Abstract: Abstract Background: Various studies have examined possible correlations between a number of cytokine gene polymorphisms and periodontal disease in populations of different origins. The present study sought the correlation between four single-nucleotide polymorphisms (IL1A+3954, IL1B+4845, TNFA-308, COL1A1 Sp1), a variable number of tandem repeats polymorphism (IL1RN intron 2) and periodontal conditions in subjects of Greek origin. Methods: One hundred and ninety-two healthy subjects, stratified as non-periodontitis and periodontitis (chronic and aggressive) cases, participated in the present study. Genotyping was performed by polymerase chain reaction-based techniques using the primers and conditions described in the literature. The frequencies of genotypes between study groups were compared using Genepop v3.3 genetic software and Instat statistical package. Results: No differences were observed among the groups concerning the distributions of genotypes under investigation. Conclusions: Carriage rates of the polymorphisms under investigation in systemically healthy subjects of Greek origin are well within the range reported for Caucasians but these polymorphisms cannot discriminate between non-periodontitis and periodontitis (chronic or aggressive) cases. Author Affiliation: (1)Department of Preventive Dentistry, Periodontology, and Implant Biology, Dental School, Aristotle University of Thessaloniki, Thessaloniki, Greece (2)Department of Genetics, Development, and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece Article History: Accepted for publication 25 June 2006 Article note: Address:, Dimitra Sakellari, Department of Preventive Dentistry, Periodontology, and Implant Biology, Dental School, Aristotle University of Thessaloniki, Thessaloniki, Greece, E-mail: dimisak@med.auth.gr