부산대학교 도서관

Background: Pulsed radiofrequency (PRF) is beneficial for radicular pain and is commonly administered at pulse frequencies of 2 or 4 Hz. However, its effects on healthy neurons have not yet been widely studied. This study aims to determine the effect of PRF at 2 Hz and 4 Hz on the physiology of healthy dorsal root ganglion (DRG) neurons. Methods: An in vitro experimental study was conducted using DRG neuron cultures divided into three groups. Control cells received no treatment, one cell group received 20 ms 2 Hz PRF for 360 s, and one cell group received a 4 Hz PRF 10 ms pulse for 360 s with similar energy. [Ca.sup.2+] influx, mitochondrial membrane potential ([DELTA][psi]m), cytosolic Adenosine triphosphate (ATP), and phosphorylated extracellular signal-regulated kinase (pERK) levels were measured. The data were analyzed using the One-Way ANOVA variance with [alpha]=5%. Results: DRG neurons exposed to PRF 2 Hz did not experience a significant change in [Ca.sup.2+] influx, whereas PRF 4 Hz caused a significant decrease in [Ca.sup.2+] influx compared to the basal level. PRF at 2 Hz did not cause a change in [DELTA][psi]m, whereas PRF at 4 Hz caused a significant decrease in [DELTA][psi]m (p0.05), indicating that there was no significant neuron activation. Conclusion: Both frequencies did not significantly activate DRG neurons, but with similar energy delivery, PRF 2 Hz preserved the physiological properties of healthy neurons better than PRF 4 Hz did. A 2 Hz PRF is the preferred frequency in clinical applications for neuron-targeted therapy. Keywords: pulsed radiofrequency, neurons, physiology, dorsal root ganglion neuron
Introduction Neuropathic pain is a type of chronic pain caused by damage to the peripheral nerves or the central nervous system. Radicular pain occurs due to the stimulation of the [...]