부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/S0723-2020(00)80040-5 Byline: Marleen Van Looveren (1), Peter Vandamme (1)(2), Wim Wuyts (3), Margaretha Ieven (1), Herman Goossens (1) Keywords: ARDRA; Neisseria gonorrhoeae; 16S-23S gene spacer region; sequence analysis Abstract: Ribosomal rRNA gene fragments (rDNA) encompassing part of the 16S rDNA, the 16S-23S rDNA spacer region and part of the 23S rDNA of 229 Neisseria gonorrhoeae strains were enzymatically amplified using conserved primers. The fragments of approximately 1200 bp were subjected to restriction analysis with HinfI. This revealed 13 patterns (patterns I-XIII) of which patterns I (78 strains), II (32 strains), III (38 strains) and IV (56 strains) were the most abundant, comprising 89.1% of the strains. The obtained restriction patterns consisted of 3 to 8 bands, ranging in size from 32 to 854 bp. The sum of the obtained bands was about 1200 bp for patterns I, II, III, IV, V, IX, and XIII. However, for patterns VI, VII, VIII, X, XI and XII, the sum of the bands well exceeded the estimated size of approximately 1200 bp. We demonstrated that this results from sequence divergence in the 4 rRNA operons, present in the genome of N. gonorrhoeae, giving rise to patterns that are a combination of several other patterns. Author Affiliation: (1) Department of Medical Microbiology, University Hospital Antwerp, University of Antwerp, Antwerp, Belgium (2) Laboratory of Microbiology, Faculty of Sciences, University of Ghent, Ghent, Belgium (3) Department of Medical Genetics, University of Antwerp, Antwerp, Belgium Article History: Received 19 January 2000