부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jchromb.2005.08.025 Byline: Giuseppe Carlucci (a), Dorina Di Pasquale (b), Fabrizio Ruggieri (b), Pietro Mazzeo (b) Keywords: Pesticides; HPLC; Human urine; SPE Abstract: A method based on solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) was developed for the simultaneous determination of 3-(3,5-diclorophenyl)-5-ethenyl-5-methyl-2,4-oxazolidinedione (vinclozolin) and 3-(3,5-diclorophenyl)-N-(1-methylethyl)-2,4-dioxo-1-imidazolidinecarboxamide (iprodione) in human urine. Urine samples containing vinclozolin and iprodione were collected by solid phase extraction using C.sub.18 cartridges. The chromatographic separation was achieved on a Spherisorb ODS2 (250mmx4.6mm, 5[mu]m) column with an isocratic mobile phase of acetonitrile-water (60:40, v/v). Detection was UV absorbance at 220nm. The calibration graphs were linear from 30 to 1000ng/mL for the two fungicides. Intra- and inter-day R.S.D. did not exceed 2.9%. The quantitation limit was 50ng/mL for vinclozolin and 30ng/mL for iprodione, respectively. Author Affiliation: (a) Dipartimento di Scienze del Farmaco, Facolta di Farmacia, Universita degli Studi "G. D'Annunzio" Chieti, Via dei Vestini, 66100 Chieti, Italy (b) Dipartimento di Chimica, Ingegneria Chimica e Materiali, Universita degli Studi dell'Aquila, Via Vetoio, 67010 Coppito (L'Aquila), Italy Article History: Received 1 October 2004; Accepted 16 August 2005