학술논문
Nodulation and effective nitrogen fixation of Macroptilium atropurpureum (siratro) by Burkholderia tuberum, a modulating and plant growth promoting beta-proteobacterium, are influenced by environmental factors
Document Type
Report
Author
Source
Plant and Soil. August 1, 2013, Vol. 369 Issue 1-2, 543
Subject
Language
English
ISSN
0032-079X
Abstract
Introduction Burkholderia tuberum [STM678.sup.T], the major focus of this study, and other nitrogen-fixing members of the Burkholderiaceae have been isolated not only from a broad range of plant species, but [...]
Background and aims Burkholderia tuberum [STM678.sup.T] was isolated from a South African legume, but did not renodulate this plant. Until a reliable host is found, studies on this and other interesting beta-rhizobia cannot advance. We investigated B. tuberum [STM678.sup.T,] sability to induce [Fix.sup.+] nodules on a small-seeded, easy-to-propagate legume (Macroptilium atropurpureum). Previous studies demonstrated that B. tuberum elicited either [Fix.sup.-] or [Fix.sup.+] nodules on siratro, but the reasons for this difference were unexplored. Methods Experiments to promote effective siratro nodule formation under different environmental conditions were performed. B. tuberum [STM678.sup.T]'s ability to withstand high temperatures and desiccation was checked as well as its potential for promoting plant growth via mechanisms in addition to nitrogen fixation, e.g., phosphate solubilization and siderophore production. Potential genes for these activities were found in the sequenced genomes. Results Higher temperatures and reduced watering resulted in reliable, effective nodulation on siratro. Burkholderia spp. solubilize phosphate and produce siderophores. Genes encoding proteins potentially involved in these growth-promoting activities were detected and are described. Conclusions Siratro is an excellent model plant for B. tuberum [STM678.sup.T]. We identified genes that might be involved in the ability of diazotrophic Burkholderia species to survive harsh conditions, solubilize phosphate, and produce siderophores. Keywords Siratro * Burkholderia * Abiotic stress * Phosphate solubilization * Siderophore
Background and aims Burkholderia tuberum [STM678.sup.T] was isolated from a South African legume, but did not renodulate this plant. Until a reliable host is found, studies on this and other interesting beta-rhizobia cannot advance. We investigated B. tuberum [STM678.sup.T,] sability to induce [Fix.sup.+] nodules on a small-seeded, easy-to-propagate legume (Macroptilium atropurpureum). Previous studies demonstrated that B. tuberum elicited either [Fix.sup.-] or [Fix.sup.+] nodules on siratro, but the reasons for this difference were unexplored. Methods Experiments to promote effective siratro nodule formation under different environmental conditions were performed. B. tuberum [STM678.sup.T]'s ability to withstand high temperatures and desiccation was checked as well as its potential for promoting plant growth via mechanisms in addition to nitrogen fixation, e.g., phosphate solubilization and siderophore production. Potential genes for these activities were found in the sequenced genomes. Results Higher temperatures and reduced watering resulted in reliable, effective nodulation on siratro. Burkholderia spp. solubilize phosphate and produce siderophores. Genes encoding proteins potentially involved in these growth-promoting activities were detected and are described. Conclusions Siratro is an excellent model plant for B. tuberum [STM678.sup.T]. We identified genes that might be involved in the ability of diazotrophic Burkholderia species to survive harsh conditions, solubilize phosphate, and produce siderophores. Keywords Siratro * Burkholderia * Abiotic stress * Phosphate solubilization * Siderophore