학술논문
Comparison of two stationary phases for the separation of five selected polyphenols
Document Type
Report
Source
Talanta. Oct 19, 2008, Vol. 77 Issue 1, p98, 5 p.
Subject
Language
English
ISSN
0039-9140
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.talanta.2008.05.044 Byline: A. Rodriguez-Bernaldo de Quiros, M.A. Lage-Yusty, J. Lopez-Hernandez Keywords: Stationary phases; Polyphenols; Performance characteristics; Method validation; HPLC Abstract: In this study, a conventional TEKNOKROMA, Tr-015605 TRACER EXTRASIL ODS2 (25cmx0.4cm, i.d. 5[mu]m) column and a new stationary phase TEKNOKROMA, Tr-010065 Mediterranea sea.sub.18 (15cmx0.4cm, i.d. 3[mu]m) were compared for the separation of five phenolic compounds. Sensitivity, performance characteristics, analysis time and method validation were reported for both columns. A better sensitivity and performance were achieved with the novel chromatographic support. In addition the total analysis time was significantly reduced. The method was applied to the determination of the selected polyphenols in commercial available white wines. LC-MS was used as a confirmatory technique. Author Affiliation: Analytical Chemistry, Nutrition and Bromatology Department, Pharmacy Faculty, Campus Sur s/n, University of Santiago de Compostela, 15782 Santiago de Compostela (La CoruA[+ or -]a), Spain Article History: Received 6 February 2008; Revised 19 May 2008; Accepted 29 May 2008