부산대학교 도서관

Aim: This research suggested an in vitro virucidal action of a dental gel and a mouthwash with phthalocyanine derivative. Purpose: The aim of this study was to report an in vitro study evaluating the virucidal capacity of mouthwash and dental gel containing anionic phthalocyanine derivate (APD). Methods: The research followed the recommendations of the National Health Surveillance Agency (ANVISA) and adapted methodology, described in the standards EN14776: 2015; ASTM E1053-11 and the Robert Koch Institute--RKI, in addition to good laboratory practices (GLP). The determination of the percentage of inactivation of the SARS-CoV-2 virus particles was carried out by imposing the viral solution in contact with the respective tested products, with intervals of 30 seconds, 1 and 5 minutes, with subsequent submission of the aliquots, recovered in cell culture microplates following virus titration using the [TCID.sub.50] (50% Median Tissue Culture Infectious Dose). Results: The Mouthwash APD presented 90% of viral inactivation percentage, while the dental gel APD demonstrated 99.99% of viral inactivation. Conclusion: In vitro analyses showed that mouthwash and dental gel APD can reduce the viability of SARS-CoV-2 virus particles. Keywords: mouthwash, dentifrices, coronavirus infections, in vitro techniques
Introduction Phthalocyanines are chemical compounds widely used as photosensitizing dyes in photodynamic therapy (PDT). The antimicrobial property of phthalocyanines has been demonstrated in several in vitro studies in the literature, [...]