학술논문
AIF, PARP-1 and MicroRNA-9 Expression in Cerebral Ischemia Associated to Alcoholism Experimental Model/Expresion de AIF, PARP-1 y MicroRNA-9 de Isquemia Cerebral Asociado a un Modelo Experimental de Alcoholismo
Document Type
Periodical
Author
Abrahao, Dayana Pousa Siqueira; Neto, Fermino Sanches Lizarte; Rodrigues, Andressa Romualdo; de Assis Cirino, Mucio Luiz; da Silva, Jairo Pinheiro; Sarraipo, Vagner Schiavoni; de Carvalho, Camila Albuquerque Melo; Tazima, Maria de Fatima Galli Sorita; Carlotti, Carlos Gilberto, Jr.; Colli, Benedicto Oscar; Tirapelli, Luis Fernando; da Cunha Tirapelli, Daniela Pretti
Source
International Journal of Morphology. September, 2020, Vol. 38 Issue 3, p616, 6 p.
Subject
Language
English
ISSN
0717-9367
Abstract
INTRODUCTION Cerebral ischemia is one of the most serious cerebrovascular diseases, causing morbidity and mortality worldwide (Donnan et al., 2008; Mandel et al., 2012). The use of alcoholic beverages is [...]
The chronic consumption of alcohol causes a worsening of the events that follow the cerebral ischemia. These events are regulated through the expression of several genes and microRNAs. The aimof this work was To analyze and describe the expression profile of PARP and AIF and miRNA-9 proteins in rats submitted to focal cerebral ischemia, associated or not with chronic alcoholism model. Methods: Twenty adult Wistar rats, subdivided into: control; ischemic; alcoholic and ischemic / alcoholized for immunohistochemical analysis and miRNA-9 gene expression. Results: There was a reduction in the protein expression of PARP-1 and a positive marking for AIF in the ischemic / alcoholized group. The miRNA-9 did not obtain significant expression. The association of ischemia with chronic alcohol use promoted a tendency to low expression of miRNA-9, low expression of PARP-1 and high expression of AIF, indicating an interference in the protective effect of miRNA-9 be observed in the other groups. KEY WORDS: PARP-1; AIF; miRNA-9; Cerebral ischemia; Alcoholism.
The chronic consumption of alcohol causes a worsening of the events that follow the cerebral ischemia. These events are regulated through the expression of several genes and microRNAs. The aimof this work was To analyze and describe the expression profile of PARP and AIF and miRNA-9 proteins in rats submitted to focal cerebral ischemia, associated or not with chronic alcoholism model. Methods: Twenty adult Wistar rats, subdivided into: control; ischemic; alcoholic and ischemic / alcoholized for immunohistochemical analysis and miRNA-9 gene expression. Results: There was a reduction in the protein expression of PARP-1 and a positive marking for AIF in the ischemic / alcoholized group. The miRNA-9 did not obtain significant expression. The association of ischemia with chronic alcohol use promoted a tendency to low expression of miRNA-9, low expression of PARP-1 and high expression of AIF, indicating an interference in the protective effect of miRNA-9 be observed in the other groups. KEY WORDS: PARP-1; AIF; miRNA-9; Cerebral ischemia; Alcoholism.