학술논문
Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
Document Type
Periodical
Author
Source
Veterinary World. March 2019, Vol. 12 Issue 3, p434, 6 p.
Subject
Language
English
ISSN
0972-8988
Abstract
Background: Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry. Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains. Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine speciesspecific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles. Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur(CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprimsulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF. Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates. Keywords: antibiotics susceptibility profiles, molecular characterization, Pasteurella multocida, pig.
Introduction Progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs are the main diseases caused by Pasteurella multocida (Pm) [1]. This agent is part of the porcine respiratory complex [...]
Introduction Progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs are the main diseases caused by Pasteurella multocida (Pm) [1]. This agent is part of the porcine respiratory complex [...]