부산대학교 도서관

This study aimed to determine the protective effects of tetrandrine (Tet) on murine ischemia-injured retinal ganglion cells (RGCs). For this, we used serum deprivation cell model, glutamate and hydrogen peroxide ([H.sub.2][O.sub.2])-induced RGC-5 cell death models, and staurosporine-differentiated neuron-like RGC-5 in vitro. We also investigated cell survival of purified primary-cultured RGCs treated with Tet. An in vivo retinal ischemia/reperfusion model was used to examine RGC survival after Tet administration 1 day before ischemia. We found that Tet affected RGC-5 survival in a dose- and time-dependent manner. Compared to dimethyl sulfoxide treatment, Tet increased the numbers of RGC-5 cells by 30% at 72 hours. After 48 hours, Tet protected staurosporine-induced RGC-5 cells from serum deprivation-induced cell death and significantly increased the relative number of cells cultured with 1 mM [H.sub.2][O.sub.2] (P Keywords: RGC-5, serum deprivation, glaucoma, mitochondrial membrane potential, apoptosis
Introduction Tetrandrine (Tet) is a bisbenzylisoquinoline alkaloid extracted from the root of the Chinese herb creeper Stephania tetrandra. (1,2) It is a calcium channel blocker (3,4) that inhibits lipid peroxidation5 [...]