부산대학교 도서관

Introduction The microtubule cytoskeleton is essential for almost all cellular activities. Microtubules themselves are the main components of important structures, such as spindles, axons and centrosomes, and mutations in tubulin-encoding [...]
Microtubule-severing proteins (MTSPs) are a group of microtubule-associated proteins essential for multiple microtubule-related processes, including mitosis and meiosis. Katanin p60 ATPase-containing subunit A-like 1 (p60 katanin-like 1) is an MTSP that maintains the density of spindle microtubules at the poles in mitotic cells; however, to date, there have been no studies about its role in female meiosis. Using in vitro-matured (IVM) oocytes as a model, it was first revealed that p60 katanin-like 1 was predominant in the ovaries and oocytes, indicating its essential roles in oocyte meiosis. It was also revealed that p60 katanin-like 1 was concentrated at the spindle poles and co-localized and interacted with y-tubulin, indicating that it may be involved in pole organization. Next, specific siRNA was used to deplete p60 katanin-like 1; the spindle organization was severely disrupted and characterized by an abnormal width:length ratio, multipolarity and extra aster microtubules out of the main spindles. Finally, it was determined that p60 katanin-like 1 knockdown retarded oocyte meiosis, reduced fertilization, and caused abnormal mitochondrial distribution. Collectively, these results indicated that p60 katanin-like 1 is essential for oocyte meiosis by ensuring the integrity of the spindle poles. Key words: microtubule-severing proteins, katanin p60 ATPa-secontaining subunit A-like 1, oocytes, meiosis, spindle