부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.gene.2005.10.005 Byline: Shauna J. Alexander, Nathaniel S. Woodling, Barry Yedvobnick Keywords: P element; Modifier screen; Wing Abbreviations: EP, Enhancer Promoter; Rp, Ribosomal protein; mM, millimolar; PBS, phosphate buffered saline; DAB, Diaminobenzidine; SDS, sodium dodecyl sulfate; TE, Tris-EDTA; PCR, polymerase chain reaction Abstract: We have utilized a transposable P element construct to scan the genome for modifiers of Drosophila Notch pathway phenotypes. From a collection of 2000 inserts we obtained two enhancers and one suppressor. Sequence analysis of the insertion regions demonstrated that two modifiers affect known components of the Notch pathway, whereas the third is an insert in the gene encoding ribosomal protein L13a at cytogenetic region 83B6-7. The insert in the RpL13A coding region creates a classic Minute mutation which enhances Notch pathway wing phenotypes. This report adds RpL13A to the list of Drosophila ribosomal protein genes that cause Minute phenotypes when mutated. Author Affiliation: Department of Biology, Emory University, Rollins Research Building, 1510 Clifton Road, Atlanta, GA. 30322, United States Article History: Received 21 June 2005; Revised 8 September 2005; Accepted 6 October 2005 Article Note: (miscellaneous) Received by J. Deutsch