학술논문

Characteristics and metabolic pathway of acetamiprid biodegradation by Fusarium sp. strain CS-3 isolated from soil
Document Type
Report
Source
Biodegradation. December, 2018, Vol. 29 Issue 6, p593, 11 p.
Subject
Metabolites -- Physiological aspects -- Analysis
Biodegradation -- Physiological aspects -- Analysis
Language
English
ISSN
0923-9820
Abstract
An acetamiprid-degrading fungus was isolated from contaminated soil and identified as Fusarium sp. strain CS-3 based on physiological, biochemical, and molecular analyses. Strain CS-3 exploited 50 mg/L as the sole carbon source in liquid culture, removing 98% in 96 h. Strain CS-3 retained its acetamiprid degradation abilities over a wide range of pH (5.0-8.0) and temperature (20-42 °C). HPLC-MS analysis showed that N'-[(6-chloropyridin-3-yl)methyl]-N-methylacetamide, 2-chloro-5-hydroxymethylpyridine, and 6-chloronicotinic acid were identified as the most predominant metabolites, forming the basis for a newly described acetamiprid degradation pathway. Strain CS-3 efficiently degraded 99.6% of 50 mg/kg acetamiprid in soil, indicating potential for soil remediation. Graphical abstract
Author(s): Zhoukun Shi [sup.1], Weiliang Dong [sup.1] [sup.2], Fengxue Xin [sup.1] [sup.2], Jiawei Liu [sup.1], Xinhai Zhou [sup.1], Fanli Xu [sup.1], Ziyao Lv [sup.1], Jiangfeng Ma [sup.1] [sup.2], Wenming Zhang [...]