부산대학교 도서관

Convenient techniques for measuring rates of bone turnover have been developed in recent years with the advent of biochemical markers of bone metabolism. One recent of these techniques is a collection method and quantitative enzyme immunoassay for free pyridinoline crosslinks in human sweat. The concentrations of pyridinoline crosslinks in 5-day sweat collections and first morning void and 24-hour urine collections from healthy subjects and subjects with established metabolic bone disorders were determined. T-scores were higher in the sweat system than in the urine system by up to 10-fold in postmenopausal subjects, women with hyperparathyroidism, and subjects with postmenopausal osteoporosis. For subjects with postmenopausal osteoporosis, receiver-operating characteristic curve analysis yielded areas under the curve of 0.699, 0.629, and 0.520 for sweat pyridinoline, first morning void urine pyridinoline, and 24 hour urine pyridinoline respectively. The areas under the curve of the sweat and first morning void urine measurements were significantly greater (p Key words: Enzyme immunoassay; Bone markers; Pyridinoline crosslinks. Abbreviations: ALN, alendronate; AUC, area under the curve; Cr, urinary creatinine; DPD, deoxypyridinoline; EIA, enzyme immonoassay; ERT, estrogen replacement therapy; FMV, first morning void; ISE, ion selective electrode; K, sweat potassium; PYD, pyridinoline; ROC, receiver-operating characteristic.
Introduction Invasive techniques for measuring bone remodeling have been available for many years. These methods have included histomorphometry of the iliac crest (1), calcium kinetic studies (2), and whole body [...]