부산대학교 도서관

We study the L-41 KD/84, and RD cells cultured as a monolayer on a glass substrate. This work is aimed at studying the laws of variation in the speckle-dynamics signals in space and time in the image plane of a monolayer of cells for high optical magnifications, different rates of the environmental-temperature variations, exposure to other virus types, and after the cell defrosting. It is shown that at a temperature-variation rate of 0.01[degrees] C/min, a good correlation is observed between the arrays of the standard deviation [[sigma].sub.u] of the optical-path difference of the wave pairs transmitting through the object and the temperature (the correlation coefficient is 0.88). It is noted that the major part of the cultured cells is the most active in the temperature range 32-34[degrees]C, which allows us to propose this particular range for studying the processes in living cells. The value of [[sigma].sub.u], which four times exceeds the corresponding value for the cells infected by the herpes virus, is obtained for the first time on the monolayer of the cultured RD cells infected by the ECHO11 virus, which agrees with the virology data for the above-mentioned viruses. Assuming that the processes occurring in the nutrient solution and inside the cells, the cell contacts, and the chaotic movement of the cells independently change the phases of the sounding waves, an excellent coincidence (the correlation coefficient is 0.99) of the experimental and theoretical time autocorrelation functions of the radiation intensity was obtained during the experiments with defrosted cells. It is concluded that the used method is promising for studying the normally functioning cells and the cells exposed to infections, toxic substances, and medicines.
UDC 535.4+57.08 1. INTRODUCTION If a rough object is illuminated by coherent radiation, then the waves with random amplitudes and phases, which are reflected from the elementary parts (scattering centers) [...]