학술논문
Categorization of insitu breast carcinoma based on morphology and immunohistochemistry
Document Type
Report
Source
Journal of Evolution of Medical and Dental Sciences. September 29, 2014, Vol. 3 Issue 48, p11511, 9 p.
Subject
Language
English
ISSN
2278-4748
Abstract
INTRODUCTION: Insitu breast carcinomas are classified, according to their morphology, as ductal carcinoma insitu (DCIS) or lobular neoplasia (LN), which includes lobular carcinoma insitu (LCIS) and atypical lobular hyperplasia (ALH). [...]
BACKGROUND: The distinction between lobular neoplasia of the breast and ductal carcinoma insitu has important therapeutic implications. In some cases, it is very difficult to determine whether the morphology of the lesion is ductal or lobular. The aim of this study was to evaluate the value of E-cadherin and β-catenin expression through the immune phenotypical characterization of carcinoma insitu with mixed pattern (CISM). METHODS: A total of 25 cases of CISM were analyzed considering cytology/mixed architecture (ductal and lobular), nuclear pleomorphism, loss of cell cohesion, and presence of comedonecrosis. The immunophenotype pattern was considered E-cadherin positive and β-catenin positive, or negative. RESULTS: Nineteen (76%) cases presented a mixed cytology and/ or architectural pattern, two (8%) presented nuclear pleomorphism, two (8%) presented mixed cytology and nuclear pleomorphism, and two (8%) presented comedonecrosis and nuclear pleomorphism. A complete positivity for E-cadherin and β-catenin was observed in 11cases (44%). In one case, the lesion was negative for both markers and showed nuclear pleomorphism. Thirteen lesions showed negative staining in areas of lobular cytology and positive staining in cells presenting the ductal pattern. CONCLUSIONS: The expression of E-cadherin and β-catenin, combined with cytological and architectural analysis, may highlight different immunophenotypes and improve classification of CISM. KEYWORDS: E-cadherin, β-catenin, Breast cancer, Lobular neoplasia, Ductal carcinoma in situ, Immunohistochemistry.
BACKGROUND: The distinction between lobular neoplasia of the breast and ductal carcinoma insitu has important therapeutic implications. In some cases, it is very difficult to determine whether the morphology of the lesion is ductal or lobular. The aim of this study was to evaluate the value of E-cadherin and β-catenin expression through the immune phenotypical characterization of carcinoma insitu with mixed pattern (CISM). METHODS: A total of 25 cases of CISM were analyzed considering cytology/mixed architecture (ductal and lobular), nuclear pleomorphism, loss of cell cohesion, and presence of comedonecrosis. The immunophenotype pattern was considered E-cadherin positive and β-catenin positive, or negative. RESULTS: Nineteen (76%) cases presented a mixed cytology and/ or architectural pattern, two (8%) presented nuclear pleomorphism, two (8%) presented mixed cytology and nuclear pleomorphism, and two (8%) presented comedonecrosis and nuclear pleomorphism. A complete positivity for E-cadherin and β-catenin was observed in 11cases (44%). In one case, the lesion was negative for both markers and showed nuclear pleomorphism. Thirteen lesions showed negative staining in areas of lobular cytology and positive staining in cells presenting the ductal pattern. CONCLUSIONS: The expression of E-cadherin and β-catenin, combined with cytological and architectural analysis, may highlight different immunophenotypes and improve classification of CISM. KEYWORDS: E-cadherin, β-catenin, Breast cancer, Lobular neoplasia, Ductal carcinoma in situ, Immunohistochemistry.