학술논문
Cryopreservation of seeds and protocorms of rare temperate orchids
Document Type
Academic Journal
Author
Source
Russian Journal of Plant Physiology. Feb, 2007, Vol. 54 Issue 1, p121, 7 p.
Subject
Language
English
ISSN
1021-4437
Abstract
The efficiency of cryopreservation of seeds of five rare and endangered species of temperate orchids belonging to Platanthera and Dactylorhiza genera followed by their asymbiotic culture in vitro, as well as of in vitro cultured D. fuchsii protocorms (specific stage of orchid embryo development after release from the seed coat) was investigated. Germination rates of seeds after their exposure to liquid nitrogen were species-depended and could be either higher or lower than in the unfrozen control. There was no significant difference between growth rates of protocorms of the same species obtained from seeds collected in various Russia regions and cultured for 5 months. After vitrification, 9% of D. fuchsii protocorms with a larger diameter of 1200 um survived cryopreservation; however, their growth was retarded for three months when compared to control protocorms.