부산대학교 도서관

To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1365-313X.2008.03763.x Byline: Heather Knight (1,*), Sarah G. Mugford (2,[dagger]), Bekir Ulker (1), Dahai Gao (1,[double dagger]), Glenn Thorlby (2), Marc R. Knight (1) Keywords: SFR6; cold acclimation; C-box binding factor; Arabidopsis; freezing tolerance; COR genes Abstract: Summary The sfr6-1 mutant of Arabidopsis thaliana was identified previously on the basis of its failure to undergo acclimation to freezing temperatures following exposure to low positive temperatures. This failure is attributed to a defect in the pathway leading to cold on-regulated (COR) gene expression via CBF (C-box binding factor) transcription factors. We identified a region of chromosome 4 containing SFR6 by positional mapping. Fine mapping of the sfr6-1 mutation proved impossible as the locus resides very close to the centromere. Therefore, we screened 380 T-DNA lines with insertions in genes within the large region to which sfr6-1 mapped. This resulted in the identification of two further mutant alleles of SFR6 (sfr6-2 and sfr6-3); like the original sfr6-1 mutation, these disrupt freezing tolerance and COR gene expression. To determine the protein sequence, we cloned an SFR6 cDNA based on the predicted coding sequence, but this offered no indication as to the mechanism by which SFR6 acts. The SFR6 gene itself is not strongly regulated by cold, thus discounting regulation of SFR6 activity at the transcriptional level. We show that over-expression of CBF1 or CBF2 transcription factors, which constitutively activate COR genes in the wild-type, cannot do so in sfr6-1. We demonstrate that CBF protein accumulates to wild-type levels in response to cold in sfr6-1. These results indicate a role for the SFR6 protein in the CBF pathway -downstream of CBF translation. The fact that the SFR6 protein is targeted to the nucleus may suggest a direct role in modulating gene expression. Author Affiliation: (1)School of Biological and Biomedical Sciences, Durham University, South Road, Durham, DH1 3LE, UK, and (2)School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK Article History: Received 21 August 2008; revised 6 November 2008; accepted 20 November 2008; published online 5 January 2009. Article note: (*) (fax +44 191 3341201; e-mail p.h.knight@durham.ac.uk).