학술논문
HLA-Cw allele analysis by PCR-restriction fragment length polymorphism: study of known and additional alleles
Document Type
Academic Journal
Author
Source
Proceedings of the National Academy of Sciences of the United States. Sept 12, 1995, Vol. 92 Issue 19, p8803, 5 p.
Subject
Language
ISSN
0027-8424
Abstract
A PCR-restriction fragment length polymorphism (PCR-RFLP) method for the identification of the HLA-Cw sequences that encodes the HLA class I heavy chains by the digestion of amplified DNA by restriction endonucleases is described. The patterns obtained for the HLA-Cw sequences using this PCR-RFLP method are unique which makes the identification easy and fast. Using this method, six new HLA-Cw sequences have been discovered which show that another allele-sharing cluster is present in the HLA-B locus. The HLA class I heavy chains are important in immune recognition by the cells.