부산대학교 도서관

Adaptive immune responses begin with cognate antigen presentation-dependent specific interaction between T cells and antigen-presenting cells. However, there have been limited reports on the isolation and analysis of these cellular complexes of T cell-antigen-presenting cell (T/APC). In this study, we successfully isolated intact antigen-specific cellular complexes of CD8.sup.+ T/APC by utilizing a microfluidics cell sorter. Using ovalbumin (OVA) model antigen and OT-I-derived OVA-specific CD8.sup.+ T cells, we analyzed the formation of antigen-specific and antigen-non-specific T/APC cellular complexes and revealed that the antigen-specific T/APC cellular complex was highly stable than the non-specific one, and that the intact antigen-specific T/APC complex can be retrieved as well as enriched using a microfluidics sorter, but not a conventional cell sorter. The single T/APC cellular complex obtained can be further analyzed for the sequences of T cell receptor V[alpha] and V[beta] genes as well as cognate antigen information simultaneously. These results suggested that this approach can be applied for other antigen and CD8.sup.+ T cells of mice and possibly those of humans. We believe that this microfluidics sorting method of the T/APC complex will provide useful information for future T cell immunology research.
Author(s): Soichiro Kuwabara 1, Yoshihiko Tanimoto 1, Mie Okutani 1, Meng Jie 2, Yasunari Haseda 1, Yumi Kinugasa-Katayama 2, Taiki Aoshi 2,* Introduction T cells play important roles in various [...]