학술논문
RNA-Seq profiling of circular RNA in human lung adenocarcinoma and squamous cell carcinoma
RNA-sequencing
RNA-sequencing
Document Type
Report
Author
Source
Molecular Cancer. September 4, 2019, Vol. 18 Issue 1
Subject
Language
English
ISSN
1476-4598
Abstract
Author(s): Chengdi Wang[sup.1] , Shuangyan Tan[sup.2] , Wen-Rong Liu[sup.2] , Qian Lei[sup.3] , Wenliang Qiao[sup.4] , Yangping Wu[sup.3] , Xiaoqi Liu[sup.2] , Wei Cheng[sup.2] , Yu-Quan Wei[sup.2] , Yong Peng[sup.2] [...]
Emerging evidences demonstrate that circular RNAs (circRNAs) are abnormally expressed in tumors and could serve as prognostic markers for cancers. However, the expression patterns and clinical implications of circRNAs in non-small cell lung cancer (NSCLC) remain obscure. In this study, we profiled circRNA expressions in 10 pairs of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) after ribosomal RNA-depletion and RNase R digestion to enrich circRNAs. Combining five circRNA computational programs, we found that LUAD and LUSC not only share common expression patterns, but also exhibit distinct circRNA expression signatures. Moreover, the Receiver Operating Characteristic (ROC) curve analysis indicated that hsa_circ_0077837 and hsa_circ_0001821 could serve as potential biomarkers for both LUAD and LUSC, while hsa_circ_0001073 and hsa_circ_0001495 could be diagnostic/subtyping marker for LUAD and LUSC, respectively. Therefore, our findings highlight the important diagnostic potential of circRNAs in NSCLC. Keywords: Circular RNAs, Lung adenocarcinoma, Lung squamous carcinoma, circRNA sequencing
Emerging evidences demonstrate that circular RNAs (circRNAs) are abnormally expressed in tumors and could serve as prognostic markers for cancers. However, the expression patterns and clinical implications of circRNAs in non-small cell lung cancer (NSCLC) remain obscure. In this study, we profiled circRNA expressions in 10 pairs of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) after ribosomal RNA-depletion and RNase R digestion to enrich circRNAs. Combining five circRNA computational programs, we found that LUAD and LUSC not only share common expression patterns, but also exhibit distinct circRNA expression signatures. Moreover, the Receiver Operating Characteristic (ROC) curve analysis indicated that hsa_circ_0077837 and hsa_circ_0001821 could serve as potential biomarkers for both LUAD and LUSC, while hsa_circ_0001073 and hsa_circ_0001495 could be diagnostic/subtyping marker for LUAD and LUSC, respectively. Therefore, our findings highlight the important diagnostic potential of circRNAs in NSCLC. Keywords: Circular RNAs, Lung adenocarcinoma, Lung squamous carcinoma, circRNA sequencing