부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bbrc.2005.12.091 Byline: Xiaohong Zhang (a), Ayako Mitsuru (a), Koichi Igura (a), Kenji Takahashi (a), Shizuko Ichinose (b), Satoru Yamaguchi (c), Tsuneo A. Takahashi (a) Keywords: Placenta; Mesenchymal cells; Chondrogenesis; Cartilage; Tissue engineering Abstract: Human mesenchymal stem cells are currently being studied extensively because of their capability for self-renewal and differentiation to various connective tissues, which makes them attractive as cell sources for regenerative medicine. Herein we report the isolation of human placenta-derived mesenchymal cells (hPDMCs) that have the potential to differentiate into various lineages to explore the possibility of using these cells for regeneration of cartilage. We first evaluated the chondrogenesis of hPDMCs in vitro and then embedded the hPDMCs into an atelocollagen gel to make a cartilage-like tissue with chondrogenic induction media. For in vivo assay, preinduced hPDMCs embedded in collagen sponges were subcutaneously implanted into nude mice and also into nude rats with osteochondral defect. The results of these in vivo and in vitro studies suggested that hPDMCs can be one of the possible allogeneic cell sources for tissue engineering of cartilage. Author Affiliation: (a) Division of Cell Processing, The Institute of Medical Science, The University of Tokyo (IMSUT), Tokyo, Japan (b) Instrumental Analysis Research Center for Life Science, Tokyo Medical and Dental University, Tokyo, Japan (c) Yamaguchi Hospital, Nishifunabashi, Funabashi, Chiba, Japan Article History: Received 28 November 2005