학술논문
Processing of UV damage in vitro by FEN-1 proteins as part of an alternative DNA excision repair pathway
Document Type
Academic Journal
Author
Source
Biochemistry. April 13, 1999, Vol. 38 Issue 15, p4809, 9 p.
Subject
Language
ISSN
0006-2960
Abstract
An unusual ultraviolet damage endonuclease (UVDE) that starts an excision repair mechanism different from previously observed repair pathways has been detected. Oligonucleotide substrates with site-specific cyclobutane pyrimidine dimers and (6-4) photoproducts were employed to characterize this UV damage repair mechanism. After introduction of single-strand breaks at the 5' sides of the photolesions by UVDE, these intermediates became substrates for cleavage by flap endonucleases (FEN-1 proteins). Findings indicated that the two enzymatic activities, UVDE and FEN-1, belong to an alternative excised repair mechanism for UV photoproducts.