학술논문
Development and validation of a reliable LC-MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis
Document Type
Report
Source
BMC Research Notes. August 30, 2019, Vol. 12 Issue 1
Subject
Language
English
ISSN
1756-0500
Abstract
Objective The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid concentration in response to environmental changes. Results The resulting method was fully validated according to international guidelines and demonstrated good selectivity and sensitivity with minor levels of a matrix effect and high accuracy. Keywords: Liquid chromatography tandem mass-spectrometry, LC-MS/MS, Secondary metabolites, Lichen, Usnic acid, Cladonia uncialis
Author(s): Natalia Sveshnikova[sup.1] , Tao Yuan[sup.2] , Jamie M. Warren[sup.2] and Michele D. Piercey-Normore[sup.1] Introduction Usnic acid (UA) is one of the most common and most studied lichen-specific secondary metabolites [...]
Author(s): Natalia Sveshnikova[sup.1] , Tao Yuan[sup.2] , Jamie M. Warren[sup.2] and Michele D. Piercey-Normore[sup.1] Introduction Usnic acid (UA) is one of the most common and most studied lichen-specific secondary metabolites [...]