부산대학교 도서관

To potentiate the bone formation capability of bone marrow stromal cell (BMSC)/[beta]-tricalcium phosphate ([beta]-TCP) constructs, we devised an autologous plasma-based construct. We tested its effectiveness and investigated the effects of its components on a monkey ectopic bone formation model. The autologous plasma (plateletrich plasma, PRP, or platelet-poor plasma, PPP)/BMSC/[beta]-TCP construct (R group or P group) showed significantly more bone formation at 3 and 6 weeks after implantation than a conventional BMSC/[beta]-TCP construct using a culture medium (M group). There was no significant difference between the P and R groups. Moreover, the P group constructs with a 10-fold lower cell concentration yielded equivalent bone formation to the M group at 5 weeks after implantation. To elucidate the effect of fibrin and serum contained in the plasma, five constructs were prepared using the following cell vehicles: autologous serum+fibrinogen (0, 1, 4, or 16 mg/mL) or phosphate-buffered saline+fibrinogen (4 mg/mL). The serum+fibrinogen (4 mg/mL, physiological concentration of monkeys) construct showed the most abundant bone formation at 3 weeks after implantation, though at 5 weeks no statistical difference existed among the groups. Autologous plasma efficiently promoted osteogenesis of BMSCs/porous [beta]-TCP constructs, and both fibrin and serum proved to play significant roles in the mechanism.
Introduction The supply of autologous bone grafts is insufficient to repair large bone defects. Harvesting these bone grafts has risks of infection, nerve damage, and cosmetic disability. (1,2) Despite the [...]