부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.cca.2005.07.007 Byline: Eun-Young Kim (a)(b), Hisato Iwata (b), Tomoko Suda (b), Shinsuke Tanabe (b), Masao Amano (c), Nobuyuki Miyazaki (d), Evgeny A. Petrov (e) Keywords: Aryl hydrocarbon receptor; AHR nuclear translocator; TEQs; CYP1A; CYP1B; Baikal seal Abstract: Most toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related planar halogenated aromatic hydrocarbons (PHAHs) are mediated by ligand-activated aryl hydrocarbon receptor (AHR) signaling pathway. To understand the regulation mechanism of AHR and AHR nuclear translocator (ARNT) expression in wild Baikal seal (Pusa sibirica) population contaminated by PHAHs, the present study investigated hepatic mRNA expression levels of AHR and its heterodimer, ARNT genes, in association with biological index (age, gender and body weight), PHAH accumulation and expression levels of cytochrome P450 (CYP) 1A and 1B. While there was no gender difference, the AHR mRNA expression levels were increased with ages (p =0.014) and body weights (p =0.015), indicating that AHR expression might be affected by these biological factors. The AHR mRNA expression levels exhibited significant positive correlations with total TEQs and most of individual congener TEQs derived from polychorinated dibenzo-p-dioxins, dibenzofurans and non-ortho coplanar polychorinated biphenyls (PCBs), indicating the transcriptional up-regulation of AHR expression by these congeners. On the other hand, there was no significant correlation between individual TEQs from mono-ortho coplanar PCBs and AHR expression. These results imply the structure-related transcriptional activity of AHR among PHAHs congeners. AHR mRNA levels showed positive correlations with both CYP1A protein (p =0.039) and CYP1A1 mRNA expression levels (p =0.046). In contrast to AHR expression, neither the total nor individual congener TEQs influenced ARNT at the transcriptional level. ARNT mRNA showed significant negative correlations with CYP1A/1B protein (p =0.027 and p =0.006) and CYP1A1 mRNA expression levels (p =0.039), implying the existence of different transcriptional regulation between AHR and ARNT genes and negative regulation by CYP1A/1B-mediated signaling pathways. The present findings may render significant insight on the basic mechanisms underlying regulation of AHR and ARNT expressions associated with biological factors and PHAH exposure in wild mammalian populations. Author Affiliation: (a) Ehime Prefectural Institute of Public Health and Environmental Science, 8-234 Sanban-cho, Matsuyama 790-0003, Japan (b) Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan (c) International Coastal Research Center, Ocean Research Institute, The University of Tokyo, 2-106-1 Akahama, Otsuchi, Iwate 028-1102, Japan (d) Center for International Cooperation, Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan (e) The Eastern-Siberian Scientific and Production Fisheries Center, "VOSTSIBRYBCENTR", Hakhalov st. 4, Ulan-Ude, Buryatia, 670034, Russia Article History: Received 9 February 2005; Revised 28 June 2005; Accepted 6 July 2005 Article Note: (footnote) [star] The nucleotide sequence of Baikal seal ARNT has been deposited in the DDBJ/EMBL/GenBank database under accession number AB201467.