부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.vetpar.2006.03.027 Byline: Mike B. Teglas (a)(b), Nicole L. Drazenovich (a)(b), Jeff Stott (c), Janet E. Foley (a)(b) Keywords: Foothill abortion; Vector; Ornithodoros coriaceus; Cattle; Tick-borne disease Abstract: Epizootic bovine abortion (EBA), also known as "foothill abortion", is a vector borne disease of beef cattle that graze in the mountainous regions of California, southern Oregon and western Nevada transmitted by the argasid tick Ornithodoros coriaceus. Recently, the putative agent of EBA was identified as a novel Deltaproteobacter in the order Myxococcales. In this study, a TaqMan real-time PCR (TM-PCR) protocol specific to the putative EBA agent was developed. The new real-time TM-PCR assay functioned sensitively and specifically to detect pathogen DNA in field-collected O. coriaceus ticks. The assay had an analytical sensitivity of a single plasmid copy and, when evaluated with a collection of tick-borne pathogens, yielded a positive PCR-result only for the agent of EBA. Use of the TM-PCR represents an effective tool for rapid and highly sensitive assessment of environmental risk and spatial and statistical analysis to highlight areas where there may be increased risk for EBA in susceptible cattle. Author Affiliation: (a) Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA (b) Center for Vectorborne Disease, School of Veterinary Medicine, University of California, Davis, CA 95616, USA (c) Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA Article History: Received 26 January 2006; Revised 20 March 2006; Accepted 23 March 2006