학술논문
A physical map of the mouse genome
Document Type
Academic Journal
Author
Gregory, Simon G.; Sekhon, Mandeep; Schein, Jacqueline; Zhao, Shaying; Osoegawa, Kazutoyo; Scott, Carol E.; Evans, Richard S.; Burridge, Paul W.; Cox, Tony V.; Fox, Christopher A.; Hutton, Richard D.; Mullenger, Ian R.; Phillips, Kimbly J.; Smith, James; Stalker, Jim; Threadgold, Glen J.; Birney, Ewan; Wylie, Kristine; Chinwalla, Asif; Wallis, John; Hillier, LaDeana; Carter, Jason; Gaige, Tony; Jaeger, Sara; Kremitzki, Colin; Layman, Dan; Maas, Jason; McGrane, Rebecca; Mead, Kelly; Walker, Rebecca; Jones, Steven; Smith, Michael; Asano, Jennifer; Bosdet, Ian; Chan, Susanna; Chittaranjan, Suganthi; Chiu, Readman; Fjell, Chris; Fuhrmann, Dan; Girn, Noreen; Gray, Catharine; Guin, Ran; Hsiao, Letticia; Krzywinski, Martin; Kutsche, Reta; Lee, Soo Sen; Mathewson, Carrie; McLeavy, Candice; Messervier, Steve; Ness, Steven; Pandoh, Pawan; Prabhu, Anna-Liisa; Saeedi, Parvaneh; Smailus, Duane; Spence, Lorraine; Stott, Jeff; Taylor, Sheryl; Terpstra, Wesley; Tsai, Miranda; Vardy, Jill; Wye, Natasja; Yang, George; Shatsman, Sofiya; Ayodeji, Bola; Geer, Keita; Tsegaye, Getahun; Shvartsbeyn, Alla; Gebregeorgis, Elizabeth; Krol, Margaret; Russell, Daniel; Overton, Larry; Malek, Joel A.; Holmes, Mike; Heaney, Michael; Shetty, Jyoti; Feldblyum, Tamara; Nierman, William C.; Catanese, Joseph J.; Hubbard, Tim; Waterston, Robert H.; Rogers, Jane; de Jong, Pieter J.; Fraser, Claire M.; Marra, Marco; McPherson, John D.; Bentley, David R.
Source
Nature. August 15, 2002, Vol. 418 Issue 6899, p743, 8 p.
Subject
Language
English
ISSN
0028-0836
Abstract
A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16,992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51,486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.
Author(s): Simon G. Gregory [1]; Mandeep Sekhon [2]; Jacqueline Schein [3]; Shaying Zhao [4]; Kazutoyo Osoegawa [5]; Carol E. Scott [1]; Richard S. Evans [1]; Paul W. Burridge [1]; Tony [...]
Author(s): Simon G. Gregory [1]; Mandeep Sekhon [2]; Jacqueline Schein [3]; Shaying Zhao [4]; Kazutoyo Osoegawa [5]; Carol E. Scott [1]; Richard S. Evans [1]; Paul W. Burridge [1]; Tony [...]