학술논문
Large-scale production of SAGE libraries from microdissected tissues, flow-sorted cells, and cell lines
Document Type
Periodical
Author
Khattra, Jaswinder; Delaney, Allen D.; Yongjun Zhao; Siddiqui, Asim; Asano, Jennifer; McDonald, Helen; Pandoh, Pawan; Dhalla, Noreen; Prabhu; Anna-Iiisa; Ma, Kevin; Lee, Stephanie; Ally, Adrian; Tam, Angela; Sa, Danne; Rogers, Sean; Charest, David; Stott, Jeff; Zuyderdyun, Scott; Varhol, Richard; Eaves, Connie; Jones, Steven; Holt, Robert; Hirst, Martin; Hoodless, Pamela A.; Marra, Marco A.
Source
Genome Research. Jan, 2007, Vol. 17 Issue 1, 108-116
Subject
Language
English
ISSN
1088-9051
Abstract
A serial analysis of gene expression (SAGE) library construction pipeline that is devised and implemented to generate high-quality digital gene expression profiling data is described. The pipeline comprises of several previous published improvements with the modification in the SAGE protocol resulting in improved cloning and DNA sequencing efficiencies.