부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.brainres.2006.10.061 Byline: Kathryn Swann (a), Jamie Berger (a), Shane M. Sprague (b), Yimin Wu (b), Qin Lai (c), David F. Jimenez (b), Constance M. Barone (a), Yuchuan Ding (a)(b) Keywords: MMP-2; MMP-9; Cerebral edema; Thermal injury; Burn; Blood-brain barrier Abstract: Mortality after serious systemic thermal injury may be linked to significant increases in cerebral vascular permeability and edema due to blood-brain barrier (BBB) breakdown. This BBB disruption is thought to be mediated by a family of proteolytic enzymes known as matrix metalloproteinases (MMPs). The gelatinases, MMP-2 and MMP-9, digest the endothelial basal lamina of the BBB, which is essential for maintaining BBB integrity. The current study investigated whether disruption of microvascular integrity in a rat thermal injury model is associated with gelatinase expression and activity. Seventy-two adult Sprague-Dawley rats were anesthetized and submerged horizontally, in the supine position, in 100 [degrees]C (37 [degrees]C for controls) water for 6 s producing a third-degree burn affecting 60-70% of the total body surface area. Brain edema was detected by calculating water content. Real time PCR, Western blot, and zymography were used to quantify MMP mRNA, protein, and enzyme activity levels. Each group was quantified at 3, 7, 24, and 72 h post thermal injury. Brain water content was significantly increased 7 through 72 h after burn. Expression of brain MMP-9 mRNA was significantly increased as early as 3 h after thermal injury compared to controls, remained at 7 h (p Author Affiliation: (a) Plastic and Reconstructive Surgery, USA (b) Department of Neurosurgery, The University of Texas Health Science Center at San Antonio, Mail Code 7843, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA (c) Wayne State University School of Education, USA Article History: Accepted 26 October 2006