학술논문
The cell surface protein coxsackie- and adenovirus receptor (CAR) directly associates with the Ligand-of-Numb Protein-X2 (LNX2)
Document Type
Report
Author
Source
Experimental Cell Research. Sept 10, 2005, Vol. 309 Issue 1, p110, 11 p.
Subject
Language
English
ISSN
0014-4827
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.yexcr.2005.05.023 Byline: Momina Mirza (a), Elisabeth Raschperger (a), Lennart Philipson (b), Ralf F. Pettersson (a), Kerstin Sollerbrant (a) Keywords: CAR; CXADR; LNX; CTX; Adenovirus; Tight junction; PDZ domain Abstract: The coxsackievirus and adenovirus receptor (CAR) is a cell surface protein that is proposed to be involved in cell-cell adhesion. Based on a yeast two-hybrid screen, co-immunoprecipitation and binding experiments, the intracellular tail of CAR was found to interact both in vivo and in vitro with the Ligand-of-Numb Protein-X2 (LNX2). The interacting domains between the two proteins were identified by truncation analyses and affinity chromatography. CAR and LNX2 protein expression in embryonic mouse tissues was analyzed by immunohistochemistry. The results suggest that CAR is a partner in a protein complex organized at specific subcellular sites by LNX2. Author Affiliation: (a) Ludwig Institute for Cancer Research, Stockholm Branch, Karolinska Intitutet, Box 240, SE-17177 Stockholm, Sweden (b) Department of Cell and Molecular Biology, Karolinska Institutet, SE-17177 Stockholm, Sweden Article History: Received 3 March 2005; Revised 13 May 2005