학술논문
Extracellular Interaction between hCD98 and the PDZ Class II Domain of hCASK in Intestinal Epithelia
Document Type
Author abstract
Source
The Journal of Membrane Biology. Jan, 2007, Vol. 215 Issue 1, p15, 12 p.
Subject
Language
English
ISSN
0022-2631
Abstract
The extracellular domain of the glycoprotein-associated integrin hCD98 protrudes into the basolateral extracellular space of the intestine and contains a PDZ class II-binding domain (GLLLRFPYAA, amino acids 520--529). Protein-protein interaction studies in vitro as well as in human colonic sections and Caco2-BBE cells have revealed that hCD98 coimmunoprecipitated with the basolateral membrane-associated guanylate kinase hCASK and that this interaction occurred in a PDZ domain-dependent manner. These novel results, which provide the first evidence for a PDZ domain-dependent interaction between a membrane protein and an extracellular protein, open a new field of investigation related to extracellular signaling in cell biology.