부산대학교 도서관

Objective: We present a novel method for screening eye bank donor corneas using high definition optical coherence tomography (HD-OCT). This technology allows for the quantification of endothelial/Descemet membrane (En/DM) complex thickness ex vivo. Design: Prospective interventional study. Participants: Fifty-two corneal grafts from 27 donors were included in this study. Twenty additional control eyes and 11 eyes with Fuchs' endothelial corneal dystrophy were also evaluated for comparison. Methods: A custom built, high speed HD-OCT device (Envisu R2210, Bioptigen, Buffalo Grove, IL, USA) was used to obtain images, and custom-made graph-based segmentation software was used to automatically deconstruct corneal images into micro-layers. HD-OCT imaging was used to scan through the sealed sterile case of donor corneas stored in McCarey-Kaufman medium to image their En/DM complex through the center of the cornea. Results: This technology allowed for quantification of En/DM complex thickness in all donor corneas through the sealed sterile container used to transport graft tissue. Mean En/DM complex thickness of donor corneas was 17[+ or -]4 [micro]m. The difference between donor cornea En/DM thickness and that of control subjects (16[+ or -]2 [micro]m) was not statistically significant (p=0.3), suggesting that the transport container and media do not affect measurements. There was a significant difference between En/DM thickness of Fuchs' endothelial corneal dystrophy eyes (25[+ or -]5 [micro]m) and both donor corneas (p Conclusions: We have described a new technique to measure En/DM complex thickness in eye bank donor corneas stored in a sealed sterile case. This may represent a novel adjunctive approach to screen corneal grafts for early endothelial disease. Keywords: corneal transplant, Fuchs' endothelial corneal dystrophy, corneal graft screening, microscope-integrated OCT
Introduction Quantification of Descemet membrane (DM) thickness has proven to be clinically useful in vivo. DM thickening has been documented in several forms of posterior corneal pathology including Fuchs' endothelial [...]