학술논문
Mild hyperhomocysteinemia reduces the activity and immunocontent, but does not alter the gene expression, of catalytic α subunits of cerebral [Na.sup.+], [K.sup.+]-ATPase
Document Type
Academic Journal
Author
Source
Molecular and Cellular Biochemistry. June 1, 2013, Vol. 378 Issue 1-2, p91, 7 p.
Subject
Language
English
ISSN
0300-8177
Abstract
Introduction [Na.sup.+], [K.sup.+]-ATPase, or [Na.sup.+], [K.sup.+] pump, is an integral membrane protein that regulates neuronal signaling, ion homeostasis, muscle contraction, and substrate transportation of most animal cells [1]. This enzyme [...]
[Na.sup.+], [K.sup.+]-ATPase is a membrane protein which plays a key role in the maintenance of ion homeostasis that is necessary to neuronal excitability, secondary transport and neurotransmitter uptake. Mild hyperhomocysteinemia leads to several clinical manifestations and particularly cerebral diseases; however, little is known about the mechanisms of homocysteine on cerebral [Na.sup.+], [K.sup.+]-ATPase. In the present study, we investigated the effect of mild hyperhomocysteinemia on the activity, the immunocontent of catalytic subunits ([α.sub.1], [α.sub.2], and [α.sub.3]) and the gene expression of this enzyme. We used the experimental model of mild hyperhomocysteinemia that was induced by homocysteine administration (0.03 (µmol/g of body weight) twice a day, from the 30th to the 60th postpartum day. Controls received saline in the same volumes. Results showed that mild hyperhomocysteinemia significantly decreased the activity and the immunocontent of the [α.sub.1] and [α.sub.2] subunits of the [Na.sup.+], [K.sup.+]-ATPase in cerebral cortex and hippocampus of adult rats. On the other hand, we did not observe any change in levels of [Na.sup.+], [K.sup.+]-ATPase mRNA transcripts in such cerebral structures of rats after chronic exposure to homocysteine. The present findings support that the homocysteine modulates the [Na.sup.+], [K.sup.+]-ATPase and this could be associated, at least in part, with the risk to the development of cerebral diseases in individuals with mild hyperhomocysteinemia. Keywords Mild hyperhomocysteinemia * [Na.sup.+], [K.sup.+]-ATPase * Catalytic subunits * Gene expression * Cerebral structures
[Na.sup.+], [K.sup.+]-ATPase is a membrane protein which plays a key role in the maintenance of ion homeostasis that is necessary to neuronal excitability, secondary transport and neurotransmitter uptake. Mild hyperhomocysteinemia leads to several clinical manifestations and particularly cerebral diseases; however, little is known about the mechanisms of homocysteine on cerebral [Na.sup.+], [K.sup.+]-ATPase. In the present study, we investigated the effect of mild hyperhomocysteinemia on the activity, the immunocontent of catalytic subunits ([α.sub.1], [α.sub.2], and [α.sub.3]) and the gene expression of this enzyme. We used the experimental model of mild hyperhomocysteinemia that was induced by homocysteine administration (0.03 (µmol/g of body weight) twice a day, from the 30th to the 60th postpartum day. Controls received saline in the same volumes. Results showed that mild hyperhomocysteinemia significantly decreased the activity and the immunocontent of the [α.sub.1] and [α.sub.2] subunits of the [Na.sup.+], [K.sup.+]-ATPase in cerebral cortex and hippocampus of adult rats. On the other hand, we did not observe any change in levels of [Na.sup.+], [K.sup.+]-ATPase mRNA transcripts in such cerebral structures of rats after chronic exposure to homocysteine. The present findings support that the homocysteine modulates the [Na.sup.+], [K.sup.+]-ATPase and this could be associated, at least in part, with the risk to the development of cerebral diseases in individuals with mild hyperhomocysteinemia. Keywords Mild hyperhomocysteinemia * [Na.sup.+], [K.sup.+]-ATPase * Catalytic subunits * Gene expression * Cerebral structures