부산대학교 도서관

Aims: This study examined links between DNA methylation and birth weight centile (BWC), and explored the impact of genetic variation. Materials & methods: Using HumanMethylation450 arrays, we examined candidate gene-associated CpGs in cord blood from newborns with low ([less than]15th centile), medium (40-60th centile) and high ([greater than]85th centile) BWC (n = 12). Candidates were examined in an investigation cohort (n = 110) using pyrosequencing and genotyping for putative methylation-associated polymorphisms performed using standard PCR. Results: Array analysis identified 314 candidate genes associated with BWC extremes, four of which showed [greater than or equal to] 4 BWC-linked CpGs. Of these, PM20D1 and MI886 suggested genetically determined methylation levels. However, methylation at three CpGs in FGFR2 remained significantly associated with high BWC (p = 0.004-0.027). Conclusion: We identified a novel biologically plausible candidate (FGFR2) for with BWC that merits further study.
Author(s): Kim E Haworth [sup.aff1] , William E Farrell [sup.aff1] , Richard D Emes [sup.aff2] [sup.aff3] , Khaled MK Ismail [sup.aff4] , William D Carroll [sup.aff5] , Emma Hubball [sup.aff6] [...]