학술논문
Recombinant activated protein C treatment improves tissue perfusion and oxygenation in septic patients measured by near-infrared spectroscopy
Document Type
Academic Journal
Author
Source
Critical Care. November 30, 2009, Vol. 13 Issue Suppl 5
Subject
Language
English
ISSN
1364-8535
Abstract
Author(s): Abele Donati[sup.1], Michela Romanelli[sup.1], Laura Botticelli[sup.1], Agnese Valentini[sup.1], Vincenzo Gabbanelli[sup.1], Simonetta Nataloni[sup.1], Tiziana Principi[sup.1], Paolo Pelaia[sup.1], Rick Bezemer[sup.2] and Can Ince[sup.2] Introduction During sepsis, the microcirculatory and mitochondrial dysfunction [...]
Introduction The purpose was to test the hypothesis that muscle perfusion, oxygenation, and microvascular reactivity would improve in patients with severe sepsis or septic shock during treatment with recombinant activated protein C (rh-aPC) (n = 11) and to explore whether these parameters are related to macrohemodynamic indices, metabolic status or Sequential Organ Failure Assessment (SOFA) score. Patients with contraindications to rh-aPC were used as a control group (n = 5). Materials and methods Patients were sedated, intubated, mechanically ventilated, and hemodynamically monitored with the PiCCO system. Tissue oxygen saturation (StO.sub.2) was measured using near-infrared spectroscopy (NIRS) during the vascular occlusion test (VOT). Baseline StO.sub.2 (StO.sub.2 baseline), rate of decrease in StO.sub.2 during VOT (StO.sub.2 downslope), and rate of increase in StO.sub.2 during the reperfusion phase were (StO.sub.2 upslope) determined. Data were collected before (T0), during (24 hours (T1a), 48 hours (T1b), 72 hours (T1c) and 96 hours (T1d)) and 6 hours after stopping rh-aPC treatment (T2) and at the same times in the controls. At every assessment, hemodynamic and metabolic parameters were registered and the SOFA score calculated. Results The mean [+ or -] standard deviation Acute Physiology and Chronic Health Evaluation II score was 26.3 [+ or -] 6.6 and 28.6 [+ or -] 5.3 in rh-aPC and control groups, respectively. There were no significant differences in macrohemodynamic parameters between the groups at all the time points. In the rh-aPC group, base excess was corrected (P < 0.01) from T1a until T2, and blood lactate was significantly decreased at T1d and T2 (2.8 [+ or -] 1.3 vs. 1.9 [+ or -] 0.7 mmol/l; P < 0.05). In the control group, base excess was significantly corrected at T1a, T1b, T1c, and T2 (P < 0.05). The SOFA score was significantly lower in the rh-aPC group compared with the controls at T2 (7.9 [+ or -] 2.2 vs. 12.2 [+ or -] 3.2; P < 0.05). There were no differences between groups in StO.sub.2 baseline. StO.sub.2 downslope in the rh-aPC group decreased significantly at all the time points, and at T1b and T2 (-16.5 [+ or -] 11.8 vs. -8.1 [+ or -] 2.4%/minute) was significantly steeper than in the control group. StO.sub.2 upslope increased and was higher than in the control group at T1c, T1d and T2 (101.1 [+ or -] 62.1 vs. 54.5 [+ or -] 23.8%/minute) (P < 0.05). Conclusions Treatment with rh-aPC may improve muscle oxygenation (StO.sub.2 baseline) and reperfusion (StO.sub.2 upslope) and, furthermore, rh-aPC treatment may increase tissue metabolism (StO.sub.2 downslope). NIRS is a simple, real-time, non-invasive technique that could be used to monitor the effects of rh-aPC therapy at microcirculatory level in septic patients. Keywords: Severe Sepsis, Septic Patient, Sequential Organ Failure Assessment, Oxygen Consumption Rate, Sequential Organ Failure Assessment Score
Introduction The purpose was to test the hypothesis that muscle perfusion, oxygenation, and microvascular reactivity would improve in patients with severe sepsis or septic shock during treatment with recombinant activated protein C (rh-aPC) (n = 11) and to explore whether these parameters are related to macrohemodynamic indices, metabolic status or Sequential Organ Failure Assessment (SOFA) score. Patients with contraindications to rh-aPC were used as a control group (n = 5). Materials and methods Patients were sedated, intubated, mechanically ventilated, and hemodynamically monitored with the PiCCO system. Tissue oxygen saturation (StO.sub.2) was measured using near-infrared spectroscopy (NIRS) during the vascular occlusion test (VOT). Baseline StO.sub.2 (StO.sub.2 baseline), rate of decrease in StO.sub.2 during VOT (StO.sub.2 downslope), and rate of increase in StO.sub.2 during the reperfusion phase were (StO.sub.2 upslope) determined. Data were collected before (T0), during (24 hours (T1a), 48 hours (T1b), 72 hours (T1c) and 96 hours (T1d)) and 6 hours after stopping rh-aPC treatment (T2) and at the same times in the controls. At every assessment, hemodynamic and metabolic parameters were registered and the SOFA score calculated. Results The mean [+ or -] standard deviation Acute Physiology and Chronic Health Evaluation II score was 26.3 [+ or -] 6.6 and 28.6 [+ or -] 5.3 in rh-aPC and control groups, respectively. There were no significant differences in macrohemodynamic parameters between the groups at all the time points. In the rh-aPC group, base excess was corrected (P < 0.01) from T1a until T2, and blood lactate was significantly decreased at T1d and T2 (2.8 [+ or -] 1.3 vs. 1.9 [+ or -] 0.7 mmol/l; P < 0.05). In the control group, base excess was significantly corrected at T1a, T1b, T1c, and T2 (P < 0.05). The SOFA score was significantly lower in the rh-aPC group compared with the controls at T2 (7.9 [+ or -] 2.2 vs. 12.2 [+ or -] 3.2; P < 0.05). There were no differences between groups in StO.sub.2 baseline. StO.sub.2 downslope in the rh-aPC group decreased significantly at all the time points, and at T1b and T2 (-16.5 [+ or -] 11.8 vs. -8.1 [+ or -] 2.4%/minute) was significantly steeper than in the control group. StO.sub.2 upslope increased and was higher than in the control group at T1c, T1d and T2 (101.1 [+ or -] 62.1 vs. 54.5 [+ or -] 23.8%/minute) (P < 0.05). Conclusions Treatment with rh-aPC may improve muscle oxygenation (StO.sub.2 baseline) and reperfusion (StO.sub.2 upslope) and, furthermore, rh-aPC treatment may increase tissue metabolism (StO.sub.2 downslope). NIRS is a simple, real-time, non-invasive technique that could be used to monitor the effects of rh-aPC therapy at microcirculatory level in septic patients. Keywords: Severe Sepsis, Septic Patient, Sequential Organ Failure Assessment, Oxygen Consumption Rate, Sequential Organ Failure Assessment Score