학술논문
Endothelial Cell Seeding Fails to Prevent Intimal Hyperplasia Following Arterial Injury in the Rat Carotid Model
Document Type
Report
Source
Cardiovascular Drugs and Therapy. Oct, 2009, Vol. 23 Issue 5, p343, 11 p.
Subject
Language
English
ISSN
0920-3206
Abstract
Purpose Aim of the study was to evaluate the therapeutic potential of endothelial cell seeding to inhibit the neointimal response after balloon injury of the rat carotid artery. Methods and results Endothelial cells were isolated from peripheral blood after cytokine-stimulation (PB-EC), the bone marrow compartment (BM-EC), and the thoracic aorta (AO-EC) of male rats. The EC-populations were characterized by microscopy, cytofluorometry, and PCR-analysis, and displayed distinct patterns of RNA-expression of the EC-markers VEGF-receptor 1 and 2, and TIE2. 5x10.sup.5 ECs were incubated in the freshly balloon-denuded arterial bed for 30 min achieving about 70% coverage with all 3 EC-populations. However, the neointimal response 2 weeks after the procedure was significantly aggravated in the EC-seeded carotids (I/M-ratio: non-seeded Control 1.00+-0.10, PB-EC 1.53+-0.07, BM-EC 1.64+-0.12, and AO-EC 1.55+-0.14 P Conclusion Endothelial cell seeding fails to prevent intimal hyperplasia following arterial injury in the rat carotid model.