학술논문
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas/Biodegradacao do petroleo e corresistencia a antibioticos por Serratia marcescens isolada em Coari, Amazonas
Document Type
Academic Journal
Author
Source
Acta Scientiarum. Biological Sciences (UEM). Oct-Dec, 2017, Vol. 39 Issue 4, p489, 8 p.
Subject
Language
English
ISSN
1679-9283
Abstract
Introduction Bacteria from Serratia genus, Enterobacteriaceae family, are characterized as Gram-negative, facultative anaerobic and chemotrophic bacilli (Hejazi & Falkiner, 1997; Carvalho et al., 2010). They are cosmopolitan bacteria (Ashelford, Fry, [...]
Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP--redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions. Keywords: biodegradation, characterization, DCPIP, antimicrobial resistance. Serratia marcescens e um bacilo Gram-negativo, anaerobio facultativo, pertencente a familia Enterobacteriaceae. Linhagens de S. marcescens sao capazes de crescer na presenca de diferentes compostos xenobioticos, dentre eles, petroleo e metais pesados. Linhagens resistentes a xenobioticos desenvolvem concomitante resistencia a multiplos antibioticos, denominada corresistencia. A linhagem AMS212 foi submetida ao metodo colorimetrico com indicador DCPIP--redox 2,6 diclorofenol indofenol, qualitativo, em microplacas. O teste quantitativo foi realizado em frascos Erlenmeyer, acompanhando-se a mudanca de coloracao, com as leituras das absorbancias. Avaliou-se o perfil de resistencia a antibioticos pelo metodo de Kirby-Bauer. No ensaio qualitativo, a linhagem AMS212 alterou a cor do DCPIP, que passou de azul para incolor, confirmando que ocorreu biodegradacao do petroleo. No teste quantitativo, as leituras foram decrescentes, confirmando que a concentracao do DCPIP diminuiu em funcao do tempo de incubacao. O teste de susceptibilidade revelou que a linhagem AMS212 apresenta multirresistencia a quatro antibioticos diferentes. S. marcescens apresentou alto desempenho na biodegradacao do petroleo, abrindo possibilidade de utiliza-la em projetos envolvendo a remediacao de areas impactadas. A expressao do fenotipo de corresistencia a antibioticos confirma que a linhagem AMS212 e capaz de resistir a diferentes agressoes ambientais. Palavras-chave: biodegradacao, caracterizacao, DCPIP, resistencia a antimicrobianos.
Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP--redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions. Keywords: biodegradation, characterization, DCPIP, antimicrobial resistance. Serratia marcescens e um bacilo Gram-negativo, anaerobio facultativo, pertencente a familia Enterobacteriaceae. Linhagens de S. marcescens sao capazes de crescer na presenca de diferentes compostos xenobioticos, dentre eles, petroleo e metais pesados. Linhagens resistentes a xenobioticos desenvolvem concomitante resistencia a multiplos antibioticos, denominada corresistencia. A linhagem AMS212 foi submetida ao metodo colorimetrico com indicador DCPIP--redox 2,6 diclorofenol indofenol, qualitativo, em microplacas. O teste quantitativo foi realizado em frascos Erlenmeyer, acompanhando-se a mudanca de coloracao, com as leituras das absorbancias. Avaliou-se o perfil de resistencia a antibioticos pelo metodo de Kirby-Bauer. No ensaio qualitativo, a linhagem AMS212 alterou a cor do DCPIP, que passou de azul para incolor, confirmando que ocorreu biodegradacao do petroleo. No teste quantitativo, as leituras foram decrescentes, confirmando que a concentracao do DCPIP diminuiu em funcao do tempo de incubacao. O teste de susceptibilidade revelou que a linhagem AMS212 apresenta multirresistencia a quatro antibioticos diferentes. S. marcescens apresentou alto desempenho na biodegradacao do petroleo, abrindo possibilidade de utiliza-la em projetos envolvendo a remediacao de areas impactadas. A expressao do fenotipo de corresistencia a antibioticos confirma que a linhagem AMS212 e capaz de resistir a diferentes agressoes ambientais. Palavras-chave: biodegradacao, caracterizacao, DCPIP, resistencia a antimicrobianos.