학술논문
Total RNA isolation fromPicea mariana dry seed
Document Type
Report
Source
Plant Molecular Biology Reporter. March, 2004, Vol. 22 Issue 1, p93, 1 p.
Subject
Language
English
ISSN
0735-9640
Abstract
Isolating RNA from dry conifer seeds can be difficult because of a number of interfering compounds present in seeds. We describe a protocol for total RNA isolation from black spruce dry seeds, which is an adaptation of a method used for mouse myeloma tissues. The extraction relies on selective precipitation of RNA by using lithium chloride.