학술논문
The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
Document Type
Academic Journal
Source
Journal of Radiation Research. January 2020, Vol. 61 Issue 1, p68, 5 p.
Subject
Language
English
ISSN
0449-3060
Abstract
INTRODUCTION In the case of a mass-casualty radiation event, it is necessary to estimate radiation doses to hundreds of thousands of individuals [1]. On smaller scales this is done manually [...]
The cytokinesis-block micronucleus (CBMN) assay is considered to be the most suitable biodosimetry method for automation. Previously, we automated this assay on a commercial robotic biotech high-throughput system (RABiT-II) adopting both a traditional and an accelerated micronucleus protocol, using centrifugation steps for both lymphocyte harvesting and washing, after whole blood culturing. Here we describe further development of our accelerated CBMN assay protocol for use on high-throughput/high content screening (HTS/HCS) robotic systems without a centrifuge. This opens the way for implementation of the CBMN assay on a wider range of commercial automated HTS/HCS systems and thus increases the potential capacity for dose estimates following a mass-casualty radiological event. Keywords: micronucleus assay; automation; biodosimetry; high-throughput
The cytokinesis-block micronucleus (CBMN) assay is considered to be the most suitable biodosimetry method for automation. Previously, we automated this assay on a commercial robotic biotech high-throughput system (RABiT-II) adopting both a traditional and an accelerated micronucleus protocol, using centrifugation steps for both lymphocyte harvesting and washing, after whole blood culturing. Here we describe further development of our accelerated CBMN assay protocol for use on high-throughput/high content screening (HTS/HCS) robotic systems without a centrifuge. This opens the way for implementation of the CBMN assay on a wider range of commercial automated HTS/HCS systems and thus increases the potential capacity for dose estimates following a mass-casualty radiological event. Keywords: micronucleus assay; automation; biodosimetry; high-throughput