학술논문
Prednisone and meloxicam in the treatment of rats underwent to acute spinal cord injury/prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal
Document Type
Academic Journal
Author
Source
Ciencia Rural. January 1, 2015, p124, 7 p.
Subject
Language
Spanish; Castilian
ISSN
0103-8478
Abstract
INTRODUCAO A formacao precoce de especies reativas de oxigenio (EROs), tambem conhecidas como radicais livres tem sido implicada como a maior causa de degeneracao do sistema nervoso central (SNC) apos [...]
The aim of the study was investigate the use of the prednisone and meloxicam in treatment of rats underwent to the experimental model of acute spinal cord injury with 2Fr Fogarty catheter, with evaluation of the oxidative stress, neurological test and histopathological analysis of the spinal cord. Ninety rats were separated into six equal groups denominated saline control or SCG, prednisone control or PCG, meloxicam control or MCG, saline and injury or STG, prednisone and injury PTG and meloxicam and injury MTG. Each group was divide into three subgroups according to treatment time in the postoperative period of 24h, 72h and seven days. All the rats underwent laminectomy and in the groups STG, MTG and PTG, after exposure of the spinal cord it was performed a compressive spinal cord injury with a 2Fr Fogarty catheter. The SCG and STG were treated with saline, MSG and MTG, with meloxicam and PSG and PTG with prednisone. All rats were evaluated for oxidative stress, neurological tests and histopathology of the spinal cord. Neurological tests were performed with Basso, Beattie e Bresnahan score (BBB), inclined plane and deep pain 24 hours before and after surgery and repeated every 48 hours until the day of euthanasia. The groups STG, MTG and PTG in the different times were zero point in the BBB scale and three points in the inclined plane and absence of deep pain. MTG and PTG had lower catalase activity and TBARS levels when compared to the STG. In the histopathological analysis it was found Wallerian degeneration and necrosis of gray matter of intensity variation. Meloxicam and prednisone can exhibit antioxidant effect, but the necrosis and Wallerian degeneration were not stop in rats underwent to acute spinal cord injury. Key words: lipid peroxidation, catalase, neurosurgery. O objetivo deste estudo foi investigar o efeito da prednisona e do meloxicam na terapia de ratos submetidos ao modelo experimental de trauma agudo da medula espinhal, induzida pelo cateter de Fogarty 2Fr, mediante a avaliacao dos parametros de estresse oxidativo, dos testes neurologicos e do exame histopatologico da medula espinhal. Foram utilizados 90 ratos Wistar, distribuidos em seis grupos, denominados controle salina ou GCS (n=15), controle prednisona ou GCP (n=15), controle meloxicam ou GCM (n=15), trauma mais salina ou GTS (n=15), trauma mais prednisona ou GTP (n=15) e trauma mais meloxicam GTM (n=15). Cada grupo foi redistribuido em tres subgrupos de igual numero, de acordo com o tempo de tratamento no pos-operatorio de 24h, 72h e sete dias. Todos os grupos foram submetidos a laminectomia e, nos grupos GTS, GTM e GTP, apos a exposicao da medula espinhal, foi realizado o trauma medular compressivo, utilizando o cateter de Fogarty 2Fr. Os grupos GCS e GTS foram tratados com solucao salina, os GSM e GTM receberam meloxicam e os GSP e GTP prednisona, sendo administrados pela via intraperitoneal. Em todos os ratos, foram avaliados os parametros de estresse oxidativo, testes neurologicos e exame histopatologico da medula espinhal. Os animais dos grupos GTS, GTM e GTP, nos diferentes tempos (24h, 72h e sete dias), tiveram pontuacao zero na escala de Basso, Beattie e Bresnahan (BBB); no plano inclinado, permaneceram com pontuacao tres e perderam a percepcao da dor profunda. Os grupos GTM e GTP apresentaram menor atividade da catalase e de niveis de TBARS, quando comparado ao grupo GTS. Foi constatada degeneracao Walleriana e necrose da substancia cinzenta de intensidades variaveis, nao apresentando diferenca entre os grupos submetidos ao trauma. O meloxicam e a prednisona apresentam possivel efeito antioxidante, mas nao impedem a necrose e a degeneracao Walleriana da medula espinhal de ratos. Palavras-chave: peroxidacao lipidica, catalase, neurocirurgia.
The aim of the study was investigate the use of the prednisone and meloxicam in treatment of rats underwent to the experimental model of acute spinal cord injury with 2Fr Fogarty catheter, with evaluation of the oxidative stress, neurological test and histopathological analysis of the spinal cord. Ninety rats were separated into six equal groups denominated saline control or SCG, prednisone control or PCG, meloxicam control or MCG, saline and injury or STG, prednisone and injury PTG and meloxicam and injury MTG. Each group was divide into three subgroups according to treatment time in the postoperative period of 24h, 72h and seven days. All the rats underwent laminectomy and in the groups STG, MTG and PTG, after exposure of the spinal cord it was performed a compressive spinal cord injury with a 2Fr Fogarty catheter. The SCG and STG were treated with saline, MSG and MTG, with meloxicam and PSG and PTG with prednisone. All rats were evaluated for oxidative stress, neurological tests and histopathology of the spinal cord. Neurological tests were performed with Basso, Beattie e Bresnahan score (BBB), inclined plane and deep pain 24 hours before and after surgery and repeated every 48 hours until the day of euthanasia. The groups STG, MTG and PTG in the different times were zero point in the BBB scale and three points in the inclined plane and absence of deep pain. MTG and PTG had lower catalase activity and TBARS levels when compared to the STG. In the histopathological analysis it was found Wallerian degeneration and necrosis of gray matter of intensity variation. Meloxicam and prednisone can exhibit antioxidant effect, but the necrosis and Wallerian degeneration were not stop in rats underwent to acute spinal cord injury. Key words: lipid peroxidation, catalase, neurosurgery. O objetivo deste estudo foi investigar o efeito da prednisona e do meloxicam na terapia de ratos submetidos ao modelo experimental de trauma agudo da medula espinhal, induzida pelo cateter de Fogarty 2Fr, mediante a avaliacao dos parametros de estresse oxidativo, dos testes neurologicos e do exame histopatologico da medula espinhal. Foram utilizados 90 ratos Wistar, distribuidos em seis grupos, denominados controle salina ou GCS (n=15), controle prednisona ou GCP (n=15), controle meloxicam ou GCM (n=15), trauma mais salina ou GTS (n=15), trauma mais prednisona ou GTP (n=15) e trauma mais meloxicam GTM (n=15). Cada grupo foi redistribuido em tres subgrupos de igual numero, de acordo com o tempo de tratamento no pos-operatorio de 24h, 72h e sete dias. Todos os grupos foram submetidos a laminectomia e, nos grupos GTS, GTM e GTP, apos a exposicao da medula espinhal, foi realizado o trauma medular compressivo, utilizando o cateter de Fogarty 2Fr. Os grupos GCS e GTS foram tratados com solucao salina, os GSM e GTM receberam meloxicam e os GSP e GTP prednisona, sendo administrados pela via intraperitoneal. Em todos os ratos, foram avaliados os parametros de estresse oxidativo, testes neurologicos e exame histopatologico da medula espinhal. Os animais dos grupos GTS, GTM e GTP, nos diferentes tempos (24h, 72h e sete dias), tiveram pontuacao zero na escala de Basso, Beattie e Bresnahan (BBB); no plano inclinado, permaneceram com pontuacao tres e perderam a percepcao da dor profunda. Os grupos GTM e GTP apresentaram menor atividade da catalase e de niveis de TBARS, quando comparado ao grupo GTS. Foi constatada degeneracao Walleriana e necrose da substancia cinzenta de intensidades variaveis, nao apresentando diferenca entre os grupos submetidos ao trauma. O meloxicam e a prednisona apresentam possivel efeito antioxidante, mas nao impedem a necrose e a degeneracao Walleriana da medula espinhal de ratos. Palavras-chave: peroxidacao lipidica, catalase, neurocirurgia.