학술논문
Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma
Document Type
Report
Author
Source
Bosnian Journal of Basic Medical Sciences. August 2013, Vol. 13 Issue 3, p186, 6 p.
Subject
Language
English
ISSN
1512-8601
Abstract
INTRODUCTION Oral squamous cell carcinoma (OSCC) is one of the most common oral cancers with increasing risk in younger people less than 40 years of age, especially in developed countries. [...]
Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P KEY WORDS: cytotoxicity, apoptosis, Anticancer, oral squamous cell carcinoma, grape seed extract
Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P KEY WORDS: cytotoxicity, apoptosis, Anticancer, oral squamous cell carcinoma, grape seed extract