학술논문
Production of [beta]-(pyrazol-1-yl)-l-alanine from l-serine and pyrazol using recombinant Escherichia coli cells expressing serine acetyltransferase and O-acetylserine sulfhydrylase-A
Document Type
Author abstract
Author
Source
Biotechnology Letters. Dec, 2001, Vol. 23 Issue 23, p2051, 5 p.
Subject
Language
English
ISSN
0141-5492
Abstract
[beta]-(Pyrazol-1-yl)-l-alanine ([beta]-PA) was produced from l-serine and pyrazol using recombinant Escherichia coli cells expressing serine acetyltransferase and O-acetylserine sulfhydrylase-A. The amount of [beta]-PA increased with increasing l-serine concentrations up to 600 mM at 50 mM pyrazol while 100 mM pyrazol gave the highest [beta]-PA production with 50 mM l-serine. Under the optimized conditions, [beta]-PA accumulated in the broth at approximately 140 mM with a conversion of 90% with respect to the added amount of pyrazol.