부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.exppara.2007.06.010 Byline: Hyun-Ouk Song (a), Myeong-Heon Shin (b), Myoung-Hee Ahn (a), Duk-Young Min (a), Yong-Seok Kim (c), Jae-Sook Ryu (a) Keywords: Trichomonas vaginalis; Neutrophils; Apoptosis; Caspase-3; ROS; reactive oxygen species; MMP; mitochondrial membrane potential; DPI; diphenyleneiodonium chloride; NAC; N-acetyl-l-cysteine; GSH; [gamma]-Glu-Cys-Gly; z-VAD-fmk; z-Val-Ala-Asp-fmk; z-DEVD-fmk; z-Asp-Glu-Val-Asp-fmk; JC-1; 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolcarbocyanine iodide; DCF-DA; 2'7'-dichlorofluorescein-diacetate Abstract: There are many neutrophils in the vaginal discharge from women infected with Trichomonas vaginalis. The aim of our study was to determine whether human neutrophil apoptosis may be regulated by reactive oxygen species (ROS) in response to trichomonads infection. Incubation of human neutrophils with live trichomonads caused marked receptor shedding of CD16, decrease of mitochondrial membrane potential (MMP) and caspase-3 activation in human neutrophils. These proapoptotic effects of T. vaginalis on neutrophils were inhibited by pretreatment of neutrophils with an inhibitor of NADPH oxidase, diphenyleneiodonium chloride (DPI), suggesting an important role of intracellular ROS accumulation in T. vaginalis-triggered apoptosis. Indeed, large amounts of ROS levels were detected in neutrophils incubated with live trichomonads, and were also effectively inhibited by DPI. However, pan-caspase inhibitor z-VAD-fmk or caspase-3 inhibitor z-DEVD-fmk did not affect T. vaginalis-induced ROS generation in neutrophils. These results suggest that ROS-dependent caspase-3 activation plays an important role in apoptosis of human neutrophils induced by T. vaginalis. Author Affiliation: (a) Department of Parasitology, Brain Korea 21, Hanyang University College of Medicine, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea (b) Department of Parasitology, Institute of Tropical Medicine, Brain Korea 21 for Medical Science, Yonsei University College of Medicine, Shinchon-dong, Seodaemoon-gu, Seoul, 120-752, Republic of Korea (c) Department of Biochemistry and Molecular Biology, Brain Korea 21 for Medical Science, Hanyang University College of Medicine, 17 Haengdang-dong, Seongdong-gu, Seoul, 133-791, Republic of Korea Article History: Received 2 October 2006; Revised 15 June 2007; Accepted 25 June 2007