부산대학교 도서관

Transfer RNA (tRNA) splicing is essential in Saccharomyces cerevisiae as well as in humans, and many of its features are the same in both. In yeast, the final step of this process is removal of the 2' phosphate generated at the splice junction during ligation. A nicotinamide adenine dinucleatide (NAD) - dependent phosphotransferase catalyzes removal of the 2' phosphate and produces a small molecule. It is shown here that this small molecule is an NAD derivative: adenosine diphosphate (ADP) - ribose 1'-2' cyclic phosphate. Evidence is also presented that this molecule is produced in Xenopus laevis oocytes as a result of dephosphorylation of ligated tRNA.
Intron-containing tRNA genes are ubiquitous in the nuclei of eukaryotes[1]. Introns invariably occur one base 3' of the anticodon, and intron removal is an essential event. Transfer RNA splicing is [...]