학술논문
Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways
Document Type
Academic Journal
Author
Source
The Journal of Cell Biology. Feb, 1992, Vol. 116 Issue 3-4, p851, 11 p.
Subject
Language
ISSN
0021-9525
Abstract
The nuclear importof the U small nuclear ribonucleic acids (U snRNAs) was studied with regard to their response to competitors for nuclear localization signal receptor and nuclear pore complex molecules. Import of U1, U2, U4 and U5 snRNPs was competedby free trimethylguanosine cap dinucleotide (TCD) but not by the synthetic karyophile P(Lys)-BSA. The import of U6 snRNA was competed by P(Lys)-BSA but not by TCD, while the import of U3 snRNA was unaffected. The results indicate three kinetic pathways for U snRNA import into the nucleus and the need for the nuclear pore complex in the process.