부산대학교 도서관

A 55 kDa cruciferin protein has been purified and characterized from seeds of Moringa oleifera plant. Protein blast of N-terminal amino-acid sequence showed 60 % sequence similarity with cruciferin from Brassica napus. The M. oleifera protein has been crystallized applying the sitting drop method using 5 % polyethylene glycol 8,000, 38.5 % 3-methyl-1,5-pentanediol and 0.1 M sodium cacodylate pH 6.5. The crystals belonged to the P6322 hexagonal space group with cell dimensions, a = b = 98.4, c = 274.3 Å. Initial diffraction data have been collected to a resolution of 6 Å.
Author(s): Ahmed Akrem [sup.1] [sup.3], Nasser Yousef [sup.1], Afshan Begum [sup.1], Amr Negm [sup.2], Arne Meyer [sup.1], Markus Perbandt [sup.1], Friedrich Buck [sup.4], Christian Betzel [sup.1] Author Affiliations: (1) grid.9026.d, [...]